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R&D Systems mouse grn elisa kit

(A) Schematic diagram of DNA injection. 6- to 8-week old female BALB/c mice were injected subcutaneously with ALD-DNA (50 µg/mice) plus CFA at week 0, followed by two booster injections of ALD-DNA (50 µg/mice) emulsified with IFA at week 2 and week 4 after initial injection. (B) Serum anti-dsDNA IgG levels were measured by <t>ELISA</t> every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (C) 8 weeks after initial injection, glomerular immune deposition were detected by direct immunofluorescence for IgG in frozen kidney section from ALD-DNA-injected SLE mice or control mice. Representative images (magnification×200) of 8 mice are shown for each group. (D) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of 8 mice in each group. (E) The kidney score was assessed using paraffin sections stained with H&E in (D). n = 8. (F) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. (G) Serum <t>GRN</t> levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (H) The correlation between serum GRN level and kidney score in lupus model. Correlation analysis was performed by Pearson correlation analysis. Each symbol indicates an individual mouse (n = 21). (I) The correlation between serum GRN level and urine protein level in lupus model. Pearson correlation analysis was used to carry out the correlation study. Each symbol indicates an individual mouse (n = 21). *, P <0.05.

Mouse Grn Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+grn+elisa+kit/pmc03673914-92-15-19?v=R%26D+Systems
Average 90 stars, based on 4 article reviews

mouse grn elisa kit - by Bioz Stars, 2026-08

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1) Product Images from "Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization"

Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

Journal: PLoS ONE

doi: 10.1371/journal.pone.0065542

Figure Legend Snippet: (A) Schematic diagram of DNA injection. 6- to 8-week old female BALB/c mice were injected subcutaneously with ALD-DNA (50 µg/mice) plus CFA at week 0, followed by two booster injections of ALD-DNA (50 µg/mice) emulsified with IFA at week 2 and week 4 after initial injection. (B) Serum anti-dsDNA IgG levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (C) 8 weeks after initial injection, glomerular immune deposition were detected by direct immunofluorescence for IgG in frozen kidney section from ALD-DNA-injected SLE mice or control mice. Representative images (magnification×200) of 8 mice are shown for each group. (D) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of 8 mice in each group. (E) The kidney score was assessed using paraffin sections stained with H&E in (D). n = 8. (F) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. (G) Serum GRN levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (H) The correlation between serum GRN level and kidney score in lupus model. Correlation analysis was performed by Pearson correlation analysis. Each symbol indicates an individual mouse (n = 21). (I) The correlation between serum GRN level and urine protein level in lupus model. Pearson correlation analysis was used to carry out the correlation study. Each symbol indicates an individual mouse (n = 21). *, P <0.05.

Techniques Used: Injection, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Control, Staining, Bicinchoninic Acid Protein Assay

BALB/c mice were administrated intramuscularly with 100 µg/mouse pGRN or pcDNA3.1 to overexpress GRN, intravenously injected with LV-shGRN or LV-shNC (2×10 8 molecules/mouse) to down-regulate GRN expression. And 72h later, mice were then injected subcutaneously with ALD-DNA (50 µg/mouse) for total 3 times in 4 weeks. (A) The dynamics of serum GRN levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (B) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of at least 8 mice in each group. (C) The kidney score was assessed using paraffin sections stained with H&E in (B). n = 8. (D) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. *, P <0.05.

Figure Legend Snippet: BALB/c mice were administrated intramuscularly with 100 µg/mouse pGRN or pcDNA3.1 to overexpress GRN, intravenously injected with LV-shGRN or LV-shNC (2×10 8 molecules/mouse) to down-regulate GRN expression. And 72h later, mice were then injected subcutaneously with ALD-DNA (50 µg/mouse) for total 3 times in 4 weeks. (A) The dynamics of serum GRN levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (B) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of at least 8 mice in each group. (C) The kidney score was assessed using paraffin sections stained with H&E in (B). n = 8. (D) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. *, P <0.05.

Techniques Used: Injection, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Bicinchoninic Acid Protein Assay

(A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time PCR analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P <0.05.

Figure Legend Snippet: (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time PCR analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P <0.05.

Techniques Used: Real-time Polymerase Chain Reaction, Western Blot, Transfection, Control, Enzyme-linked Immunosorbent Assay

(A) Primary macrophages were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by immunoblotting. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. (B) Primary macrophages were pretreated with elastase inhibitor (100 µM) for 12 h, and then were stimulated with 50 µg/mL ALD-DNA for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by Western blot. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Left, representative western blots; Right, quantitative results, the band intensity was measured by Image J and the ratios of phospho-Akt, phospho-ERK, phospho-JNK and phospho-p38 to β-actin were calculated. Primary peritoneal macrophages were pretreated with U0126 (10 µM) (C), SP600125 (10 µM) (D), SB203580 (10 µM) (E) for 30 min, and then were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for 24 h. Cytokine expression levels of TNF-α, IL-1β and IL-10 in the supernatants of macrophages were determined by ELISA assay. Data are means ± SD of three independent experiments. *, P <0.05.

Figure Legend Snippet: (A) Primary macrophages were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by immunoblotting. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. (B) Primary macrophages were pretreated with elastase inhibitor (100 µM) for 12 h, and then were stimulated with 50 µg/mL ALD-DNA for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by Western blot. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Left, representative western blots; Right, quantitative results, the band intensity was measured by Image J and the ratios of phospho-Akt, phospho-ERK, phospho-JNK and phospho-p38 to β-actin were calculated. Primary peritoneal macrophages were pretreated with U0126 (10 µM) (C), SP600125 (10 µM) (D), SB203580 (10 µM) (E) for 30 min, and then were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for 24 h. Cytokine expression levels of TNF-α, IL-1β and IL-10 in the supernatants of macrophages were determined by ELISA assay. Data are means ± SD of three independent experiments. *, P <0.05.

Techniques Used: Purification, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

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Journal: PLoS ONE

Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

doi: 10.1371/journal.pone.0065542

Figure Lengend Snippet: (A) Schematic diagram of DNA injection. 6- to 8-week old female BALB/c mice were injected subcutaneously with ALD-DNA (50 µg/mice) plus CFA at week 0, followed by two booster injections of ALD-DNA (50 µg/mice) emulsified with IFA at week 2 and week 4 after initial injection. (B) Serum anti-dsDNA IgG levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (C) 8 weeks after initial injection, glomerular immune deposition were detected by direct immunofluorescence for IgG in frozen kidney section from ALD-DNA-injected SLE mice or control mice. Representative images (magnification×200) of 8 mice are shown for each group. (D) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of 8 mice in each group. (E) The kidney score was assessed using paraffin sections stained with H&E in (D). n = 8. (F) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. (G) Serum GRN levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (H) The correlation between serum GRN level and kidney score in lupus model. Correlation analysis was performed by Pearson correlation analysis. Each symbol indicates an individual mouse (n = 21). (I) The correlation between serum GRN level and urine protein level in lupus model. Pearson correlation analysis was used to carry out the correlation study. Each symbol indicates an individual mouse (n = 21). *, P <0.05.

Article Snippet: To assess protein levels of GRN in murine serum, ELISA assays were performed with commercial mouse GRN ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Control, Staining, Bicinchoninic Acid Protein Assay

Journal: PLoS ONE

Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

doi: 10.1371/journal.pone.0065542

Figure Lengend Snippet: BALB/c mice were administrated intramuscularly with 100 µg/mouse pGRN or pcDNA3.1 to overexpress GRN, intravenously injected with LV-shGRN or LV-shNC (2×10 8 molecules/mouse) to down-regulate GRN expression. And 72h later, mice were then injected subcutaneously with ALD-DNA (50 µg/mouse) for total 3 times in 4 weeks. (A) The dynamics of serum GRN levels were measured by ELISA every 2 weeks after initial injection. Data are means ± SD from 8 mice in each group. (B) Nephritic pathology was evaluated by H&E staining of renal tissues. Images (magnification×200) are representative of at least 8 mice in each group. (C) The kidney score was assessed using paraffin sections stained with H&E in (B). n = 8. (D) Urine protein levels of the mice were assessed by BCA Protein Assay Kit every 2 weeks. Data are means ± SD from 8 mice in each group. *, P <0.05.

Article Snippet: To assess protein levels of GRN in murine serum, ELISA assays were performed with commercial mouse GRN ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Injection, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Bicinchoninic Acid Protein Assay

Journal: PLoS ONE

Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

doi: 10.1371/journal.pone.0065542

Figure Lengend Snippet: (A–B) Primary macrophages were stimulated with increasing amounts of ALD-DNA for 24 h. mRNA levels of GRN in macrophages were analyzed by real time PCR analysis (A), and protein levels of GRN in the supernatants of macrophages were analyzed by western blot (B). Above, quantitative results of western blots, the band intensity was measured by Image J; Below, representative western blots. Similar results were obtained in three independent experiments. Data are representative of results obtained in three independent experiments. Primary peritoneal macrophages were stimulated by ALD-DNA (50 µg/mL) with GRN (5 µg/mL) for 24 h (C and F), or were pretreated with elastase inhibitor (100 µM) or DMSO (0.1%) for 12 h (D and G), and then were exposed to ALD-DNA, UnALD-DNA, or PBS for another 24 h. Macrophages were transfected with control siRNA (200 nM) or GRN siRNA (siGRN, 200 nM). 36 h posttransfection, macrophages were stimulated with PBS, UnALD-DNA or ALD-DNA (50 µg/mL) (E and H). (C–E) ELISA assay was used to analyze the levels of TNF-α, IL-1β, IL-6, IL-10, IL-12, and MCP-1 in the culture supernatants of macrophages. Data are means ± SD of three independent experiments. (F–H) Western blot analysis was used to analyze the protein levels of iNOS in macrophages. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Band intensity was measured by Image J and the ratios of iNOS to β-actin were calculated. *, P <0.05.

Article Snippet: To assess protein levels of GRN in murine serum, ELISA assays were performed with commercial mouse GRN ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Transfection, Control, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Granulin Exacerbates Lupus Nephritis via Enhancing Macrophage M2b Polarization

doi: 10.1371/journal.pone.0065542

Figure Lengend Snippet: (A) Primary macrophages were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by immunoblotting. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. (B) Primary macrophages were pretreated with elastase inhibitor (100 µM) for 12 h, and then were stimulated with 50 µg/mL ALD-DNA for the indicated time. Phospho-Akt, ERK, JNK, and p38 were detected by Western blot. Data are representative of three separate experiments. Similar results were obtained in three independent experiments. Left, representative western blots; Right, quantitative results, the band intensity was measured by Image J and the ratios of phospho-Akt, phospho-ERK, phospho-JNK and phospho-p38 to β-actin were calculated. Primary peritoneal macrophages were pretreated with U0126 (10 µM) (C), SP600125 (10 µM) (D), SB203580 (10 µM) (E) for 30 min, and then were stimulated with 50 µg/mL ALD-DNA together with 5 µg/mL purified GRN for 24 h. Cytokine expression levels of TNF-α, IL-1β and IL-10 in the supernatants of macrophages were determined by ELISA assay. Data are means ± SD of three independent experiments. *, P <0.05.

Article Snippet: To assess protein levels of GRN in murine serum, ELISA assays were performed with commercial mouse GRN ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Purification, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

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